The synbiotic-fluoride (SF) therapy enhances enamel remineralization with no
biocompatibility concerns suggests a new study published in the Journal of
Dentistry
The objectives of the present study were to examine the – a)
enamel remineralization potential of synbiotic-fluoride (SF) therapy using a
multi-species bacterial pH-cycling model; and b) cytotoxic and genotoxic
effects of SF therapy extracts.
The SF therapy group comprised of 2% arginine (Arg), 0.2%
NaF, and a probiotic Lactobacillus rhamnosus GG (LRG). The intervention groups
studied were: 1) No treatment; 2) 2% Arg; 3) 0.2% NaF; 4) LRG; 5) 2% Arg+0.2%
NaF; 6) 2% Arg+LRG; 7) 0.2% NaF+LRG; and 8) 2% Arg+0.2% NaF+LRG (SF therapy).
The enamel remineralization potential of SF therapy was investigated under
cariogenic biofilm challenge; while the cytotoxicity and genotoxicity of SF
therapy extracts were examined on HGF-1 and Chinese hamster fibroblast V79,
respectively. To determine the remineralization effect, the specimens were
subjected to mineral density (MD) assessment using micro-CT, Ca/P molar ratio
with SEM-EDX, and enamel fluoride uptake (EFU) estimates. The HGF-1
proliferation assessment was quantified using MTT/CCK-8 assays with qualitative
analysis by nuclei staining Hoechst-based fluorescence imaging. The
genotoxicity was determined by micronuclei formation test.
Results
- Mineral gain and % remineralization derived from
MD assessment for the SF therapy were significantly higher than the other
groups (p<0.05). - The % ΔCa/P for the SF and 2% Arg+0.2% NaF were
significantly higher than the other groups (p<0.05). - The SF and 2% Arg+0.2% NaF groups had the
highest EFU compared to the other groups (p<0.05). - No significant difference in the % viable HGF-1
cells were observed between the treatment interventions and no treatment group
(p>0.05). - Compared to the EMS-positive control, the
micronuclei formation for all the intervention groups was significantly lower
(p0.05).
The SF therapy enhanced enamel remineralization with no
biocompatibility concerns. With the enhanced enamel remineralization potential
discerned in the present study, the SF therapy can be used as a promising
caries-preventive agent targeted for high caries-risk individuals.
Reference:
Mohammed Nadeem BIJLE, Mohamed Mahmoud ABDALLA, Chun Hung
CHU, Cynthia Kar Yung YIU. Synbiotic-fluoride synergism on enamel
remineralization, cytotoxicity and genotoxicity, Journal of Dentistry, 2022, 104356,
ISSN 0300-5712. https://doi.org/10.1016/j.jdent.2022.104356.
Keywords
Arginine, fluoride, remineralization, synbiotictoxicity, Journal
of Dentistry, Mohammed Nadeem BIJLE, Mohamed Mahmoud ABDALLA, Chun Hung CHU,
Cynthia Kar Yung YIU